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crispr-cas9-on peut modifier ---- le genome-l'arn guide se la à l'adn ----la region --- de l'arn guide est une seqeunce constante qui recrute ----la cas9 interagit avec la sequence --- de l'adn cible, puis au complexe-clivage precis de --- nucleotides en amont de ----on peut faire un knock out ou un knock in-2 possibilités de reparation: nhej = --- --- --- --- = ajout/retrait de nucleotides pour ligature -> erreurs, --- de phase de lecture -> KO, ou bien HDR = --- --- --- -> reparation par recombinaison des zones homologes de part et d'autre du site de clivage -> transfection d'un seqeunce adn donneur = KI

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