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When 1 mole of ATP is hydrolyzed in a test tube without an enzyme, about twice as much heat is given off as when 1 mole of ATP is hydrolyzed in a cell. Which of the following best explains these observations?-The amount of heat released by a reaction has nothing to do with the free energy change of the reaction.-In the cell, the hydrolysis of ATP is coupled to other endergonic reactions.-In cells, ATP is hydrolyzed to ADP and Pi, but in the test tube it is hydrolyzed to carbon dioxide and water.-Cells are less efficient at energy metabolism than reactions that are optimized in a test tube.-Cells have the ability to store heat; this cannot happen in a test tube.
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