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In an experiment, the F1 portion of the ATP synthase was attached to a glass slide, and fluorescently labeled microfilaments were attached to its central peptide. The rotation of the ATP synthase was observed when ATP was added. Because there was no proton gradient to drive the molecular motor in the direction of ATP synthesis, ATP was hydrolyzed to ADP and Pi, and this energy caused the motor to spin. When ATP plus oligomycin was added to the slide, the rotation continued even though oligomycin can inhibit ATP hydrolysis in mitochondrial inner membrane vesicles containing ATP synthase (F1-F0). Based on your knowledge of ATP synthase, which hypothesis is supported by these observations?
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