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The cell body of neuron #2 is located in the CNS however it's myelinated axon extends into the PNS where it synapses in the autonomic ganglion (cell body in PNS) with neuron #3. The nucleotransmitter ACh is released into the synaptic cleft. Neuron #2 is the pre-ganglionic neuron and neuron #3 is the post ganglionic neuron.

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